Characterisation of Al-stimulated efflux of malate from the apices of Al-tolerant wheat roots
Identifieur interne : 000131 ( Main/Exploration ); précédent : 000130; suivant : 000132Characterisation of Al-stimulated efflux of malate from the apices of Al-tolerant wheat roots
Auteurs : RBID : ISTEX:425_1995_Article_BF00193223.pdfEnglish descriptors
Abstract
Aluminium (Al) stimulates the efflux of malate from the apices of wheat (Triticum aestivum L.) roots (Delhaize et al. 1993, Plant Physiol. 103, 695–702). The response was five to tenfold higher in Al-tolerant seedlings than Al-sensitive seedlings and the capacity for Al-stimulated malate efflux was found to co-segregate with Al tolerance in a pair of near-isogenic wheat lines differing in Al-tolerance at a single dominant locus. We have investigated this response further using excised root apices. Half-maximal efflux of malate from apices of Al-tolerant seedlings was measured with 30 μM Al in 0.2 mM CaCl2, pH 4.2, while saturating rates of 2.0 nmol·apex−1·h−1 occurred with concentrations above 100 μM Al. The stimulation of malate efflux by Al was accompanied by an increase in K+ efflux which appeared to account for electroneutrality. The greater stimulation of malate efflux from Al-tolerant apices compared to Al-sensitive apices could not be explained by differences in the activities of phosphoenolpyruvate carboxylase or NAD-malate dehydrogenase. Several other polyvalent cations, including gallium, indium and the tridecamer Al13, failed to elicit malate efflux. Aluminium-stimulated efflux of malate was correlated with the measured concentration of total monomeric Al present, and with the predicted concentrations of Al3+ and AlOH2+ ions in the solution. Several antagonists of anion channels inhibited Al-stimulated efflux of malate with the following order of effectiveness: niflumic acid≈NPPB>IAA-94≈A-9-C>ethacrynic acid. Lanthanum, chlorate, perchlorate, zinc and α-cyano-4-hydroxycinnamic acid inhibited malate release by less than 30% at 100 μM while 4,4′-diisothiocyanatostilbene-2,2′-disulphonate (DIDS) had no effect. These results suggest that the Al3+ cation stimulates malate efflux via anion channels in apical cells of Al-tolerant wheat roots.
DOI: 10.1007/BF00193223
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<record><TEI><teiHeader><fileDesc><titleStmt><title>Characterisation of Al-stimulated efflux of malate from the apices of Al-tolerant wheat roots</title><author><name>Peter R. Ryan</name><affiliation wicri:level="1"><mods:affiliation>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT</wicri:regionArea><wicri:noRegion>ACT</wicri:noRegion></affiliation></author><author><name>Emmanuel Delhaize</name><affiliation wicri:level="1"><mods:affiliation>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT</wicri:regionArea><wicri:noRegion>ACT</wicri:noRegion></affiliation></author><author><name>Peter J. Randall</name><affiliation wicri:level="1"><mods:affiliation>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT</wicri:regionArea><wicri:noRegion>ACT</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="RBID">ISTEX:425_1995_Article_BF00193223.pdf</idno><date when="1995">1995</date><idno type="doi">10.1007/BF00193223</idno><idno type="wicri:Area/Main/Corpus">000C22</idno><idno type="wicri:Area/Main/Curation">000C22</idno><idno type="wicri:Area/Main/Exploration">000131</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminium (tolerance, toxicity)</term><term>Anion channel</term><term>Malate</term><term>Root apex</term><term>Triticum</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="eng">Aluminium (Al) stimulates the efflux of malate from the apices of wheat (Triticum aestivum L.) roots (Delhaize et al. 1993, Plant Physiol. 103, 695–702). The response was five to tenfold higher in Al-tolerant seedlings than Al-sensitive seedlings and the capacity for Al-stimulated malate efflux was found to co-segregate with Al tolerance in a pair of near-isogenic wheat lines differing in Al-tolerance at a single dominant locus. We have investigated this response further using excised root apices. Half-maximal efflux of malate from apices of Al-tolerant seedlings was measured with 30 μM Al in 0.2 mM CaCl2, pH 4.2, while saturating rates of 2.0 nmol·apex−1·h−1 occurred with concentrations above 100 μM Al. The stimulation of malate efflux by Al was accompanied by an increase in K+ efflux which appeared to account for electroneutrality. The greater stimulation of malate efflux from Al-tolerant apices compared to Al-sensitive apices could not be explained by differences in the activities of phosphoenolpyruvate carboxylase or NAD-malate dehydrogenase. Several other polyvalent cations, including gallium, indium and the tridecamer Al13, failed to elicit malate efflux. Aluminium-stimulated efflux of malate was correlated with the measured concentration of total monomeric Al present, and with the predicted concentrations of Al3+ and AlOH2+ ions in the solution. Several antagonists of anion channels inhibited Al-stimulated efflux of malate with the following order of effectiveness: niflumic acid≈NPPB>IAA-94≈A-9-C>ethacrynic acid. Lanthanum, chlorate, perchlorate, zinc and α-cyano-4-hydroxycinnamic acid inhibited malate release by less than 30% at 100 μM while 4,4′-diisothiocyanatostilbene-2,2′-disulphonate (DIDS) had no effect. These results suggest that the Al3+ cation stimulates malate efflux via anion channels in apical cells of Al-tolerant wheat roots.</div></front></TEI><mods xsi:schemaLocation="http://www.loc.gov/mods/v3 file:///applis/istex/home/loadistex/home/etc/xsd/mods.xsd" version="3.4" istexId="d61809d3d8851ef6656920b2534aa4a150087cc9"><titleInfo lang="eng"><title>Characterisation of Al-stimulated efflux of malate from the apices of Al-tolerant wheat roots</title></titleInfo><name type="personal"><namePart type="given">Peter R.</namePart><namePart type="family">Ryan</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia</affiliation></name><name type="personal"><namePart type="given">Emmanuel</namePart><namePart type="family">Delhaize</namePart><role><roleTerm type="text">author</roleTerm></role><description>manny@pican.pi.csiro.au</description><affiliation>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia</affiliation></name><name type="personal"><namePart type="given">Peter J.</namePart><namePart type="family">Randall</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia</affiliation></name><typeOfResource>text</typeOfResource><genre>Original Paper</genre><originInfo><publisher>Springer-Verlag, Berlin/Heidelberg</publisher><dateCreated encoding="w3cdtf">1994-06-21</dateCreated><dateCaptured encoding="w3cdtf">1994-08-18</dateCaptured><dateValid encoding="w3cdtf">2004-07-12</dateValid><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="eng">Aluminium (Al) stimulates the efflux of malate from the apices of wheat (Triticum aestivum L.) roots (Delhaize et al. 1993, Plant Physiol. 103, 695–702). The response was five to tenfold higher in Al-tolerant seedlings than Al-sensitive seedlings and the capacity for Al-stimulated malate efflux was found to co-segregate with Al tolerance in a pair of near-isogenic wheat lines differing in Al-tolerance at a single dominant locus. We have investigated this response further using excised root apices. Half-maximal efflux of malate from apices of Al-tolerant seedlings was measured with 30 μM Al in 0.2 mM CaCl2, pH 4.2, while saturating rates of 2.0 nmol·apex−1·h−1 occurred with concentrations above 100 μM Al. The stimulation of malate efflux by Al was accompanied by an increase in K+ efflux which appeared to account for electroneutrality. The greater stimulation of malate efflux from Al-tolerant apices compared to Al-sensitive apices could not be explained by differences in the activities of phosphoenolpyruvate carboxylase or NAD-malate dehydrogenase. Several other polyvalent cations, including gallium, indium and the tridecamer Al13, failed to elicit malate efflux. Aluminium-stimulated efflux of malate was correlated with the measured concentration of total monomeric Al present, and with the predicted concentrations of Al3+ and AlOH2+ ions in the solution. Several antagonists of anion channels inhibited Al-stimulated efflux of malate with the following order of effectiveness: niflumic acid≈NPPB>IAA-94≈A-9-C>ethacrynic acid. Lanthanum, chlorate, perchlorate, zinc and α-cyano-4-hydroxycinnamic acid inhibited malate release by less than 30% at 100 μM while 4,4′-diisothiocyanatostilbene-2,2′-disulphonate (DIDS) had no effect. These results suggest that the Al3+ cation stimulates malate efflux via anion channels in apical cells of Al-tolerant wheat roots.</abstract><subject lang="eng"><genre>Key words</genre><topic>Aluminium (tolerance, toxicity)</topic><topic>Anion channel</topic><topic>Malate</topic><topic>Root apex</topic><topic>Triticum</topic></subject><relatedItem type="series"><titleInfo type="abbreviated"><title>Planta</title></titleInfo><titleInfo><title>Planta</title><subTitle>An International Journal of Plant Biology</subTitle><partNumber>Year: 1995</partNumber><partNumber>Volume: 196</partNumber><partNumber>Number: 1</partNumber></titleInfo><genre>Archive Journal</genre><originInfo><dateIssued encoding="w3cdtf">1995-03-01</dateIssued><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><subject usage="primary"><topic>Life Sciences</topic><topic>Agriculture</topic><topic>Ecology</topic><topic>Forestry</topic><topic>Plant Sciences</topic></subject><identifier type="issn">0032-0935</identifier><identifier type="issn">Electronic: 1432-2048</identifier><identifier type="matrixNumber">425</identifier><identifier type="local">IssueArticleCount: 24</identifier><recordInfo><recordOrigin>Springer-Verlag, 2004</recordOrigin></recordInfo></relatedItem><identifier type="doi">10.1007/BF00193223</identifier><identifier type="matrixNumber">Art14</identifier><identifier type="local">BF00193223</identifier><accessCondition type="use and reproduction">MetadataGrant: OpenAccess</accessCondition><accessCondition type="use and reproduction">AbstractGrant: OpenAccess</accessCondition><accessCondition type="restriction on access">BodyPDFGrant: Restricted</accessCondition><accessCondition type="restriction on access">BodyHTMLGrant: Restricted</accessCondition><accessCondition type="restriction on access">BibliographyGrant: Restricted</accessCondition><accessCondition type="restriction on access">ESMGrant: Restricted</accessCondition><part><extent unit="pages"><start>103</start><end>110</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1995</recordOrigin><recordIdentifier>425_1995_Article_BF00193223.pdf</recordIdentifier></recordInfo></mods></record>Pour manipuler ce document sous Unix (Dilib)
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